Watch as sharp protein bands appear in minutes. Quick Coomassie Stain is a pleasant product as you simply pour it onto the gel immediately after the electrophoresis without any further preparations as fixing, pre-washing or de-staining is not required. You may also contact us by e-mail for price information and ordering. BSA can also be used in other protocols that involve antibody binding including ELISA, DELFIA, flow cytometry and immunofluorescence/immunocytochemistry. Ultra pure bovine serum albumin (BSA) is used as a reagent during Western blotting. 15019SĪnimal-Free Blocking Solution (5X), 25 mlĪnimal-Free Blocking Solution (5X), 250 ml This plant-based product contains no animal-derived proteins and can be used to decrease background in place of normal goat serum, BSA, or non-fat dry milk. 9999SĪnimal-Free Blocking Solution (5X) is designed for use as a blocking reagent in chromogenic immunohistochemical assays (IHC) and western blotting. Premium quality nonfat dry milk for use as a blocking agent during Western blotting to block non-specific antibody binding and reduce background signal. Revitablot™ Western Blot Stripping Buffer IPTG (Isopropyl beta-D-thiogalactoside) InducerīCIP/NBT Membrane Alkaline Phosphatase SubstrateīCIP/TNBT Membrane Alkaline Phosphatase Substrateīlocking Buffer for Fluorescent Western BlottingĬhemiluminescent FemtoMax™ Super Sensitive HRP Substrate Use Rockland blocking buffers to boost your results today.īlocking agents available from Rockland: MB-065. Our solutions can help develop more accurate and long lasting results that drive scientific innovation. Rockland’s blocking buffer formulations reduce nonspecific binding of sample and assay components to the well while stabilizing the coated protein. Assay sensitivity is improved by utilizing the appropriate blocking buffer, which will decrease background interference and improve the signal-to-noise ratio. The following examples are a variety of good Western Blot results which detect target protein in different cell lines, such as A431, HEK293 and MCF-7.Blocking buffers are used in various applications to decrease non-specific signaling generated by non-specific binding of proteins or peptides, essentially blocking Western blot noise. Sino Biological's Western Blot antibodies have the advantage of high sensitivity, high specificity and high stability. Some of these Western Blot / WB antibodies also be confirmed with animal tissues, including mouse, rat and ferret, etc. Our Western Blot antibodies were extensively validated by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells). To perform Western Blot successfully, the quality of antibodies are one of the most important factors. If you have problems in your Western Blotting, please visit " Western Blot Troubleshooting Guide" for advice. But what are the characteristics of a good Western Blot result? Normally, it means clear and straight bands showed on a membrane. Industry Insights with Yuning Chen on Recombinant ProteinsĪ good Western Blot result is required if researchers want to avoid false positives or negatives.ExpertAnswers: Yuning Chen on Antibody Production.Universal Vaccine Advancement through AI and Recombinant Technology.Nanobodies: An Important Tool for the Next Generation of Tumor Diagnostics and Therapeutics.ExpertAnswers: Amy Sheng on Antibody Screening and Discovery.Recombinant DNA Technology and Its Impact on Drug Discovery.CAR-T Cell Therapy Development: From Personalized to off the Shelf Approaches.BioBuzz with Sino | Episode 1: ChatGPT in Biotech.
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